The CCND1 gene is located on chromosome 11q13.3. The CCND1 Break Apart FISH probe is designed to label both ends of the CCND1 gene, respectively. A 600 Kb orange probe is designed at the 3′ end of the CCND1 gene, and a 700 Kb green probe is designed at the 5′ end of the CCND1 gene.

The chromosomal translocation t(11;14)(q13;q32) represents the characteristic genetic alteration in mantle cell lymphoma (MCL) and occurs in roughly 30% of multiple myeloma (MM) cases featuring 14q32 rearrangements. Alternative translocations may join CCND1 with different genes, including IGK or IGL.