The CCND3 gene is located on chromosome 6p21.1. The CCND3 Break Apart FISH probe is designed to label both ends of the CCND3 gene, respectively. A 400 Kb orange probe is designed at the 3′ end of the CCND3 gene, and a 400 Kb green probe is designed at the 5′ end of the CCND3 gene.

Rearrangements and abnormal expression of the CCND3 gene have been observed in several types of hematological malignancies such as multiple myeloma (MM), chronic lymphocytic leukemia (CLL), acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML) and others.