The RREB1(6p25.1) probe and MYB(6q23.3) probe are located on chromosome 6 and the CCND1(11q13.3) probe is located on chromosome 11. A 680 Kb probe covering the RREB1 gene region is labeled with a red dye. A 840 Kb probe covering the MYB gene region is labeled with a golden dye. A 450 Kb probe covering the CCND1 gene region is labeled with a green dye. The CEN 6 probe, labeled with Aqua, hybridizes to the alpha satellite DNA located at the centromere of chromosome 6 (6p11.1-q11.1).

Aids in diagnosis of histologically ambiguous melanocytic lesions, distinguishing early stage acral and cutaneous melanomas from dysplastic nevi. Targets RREB1, MYB, CCND1, and centromere 6 to detect copy number aberrations. Used when morphological features alone cannot determine malignant potential, providing molecular support for challenging diagnostic decisions.