The MLL gene is located on chromosome 11q23.3. The MLL Break Apart FISH probe is designed to label both ends of the MLL gene, respectively. A 500 Kb orange probe is designed at the 3′ end of the MLL gene, and a 400 Kb green probe is designed at the 5′ end of the MLL gene.

Detects MLL (KMT2A) rearrangements at 11q23.3 in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). Shows 60-80% incidence in infant acute leukemia cases. Used for diagnosis, prognosis assessment, and disease monitoring. Assists in diagnostic aid for confirmed or suspected ALL and AML patients. Essential for identifying therapy-related leukemias and treatment stratification.