The P2RY8 gene is located on chromosome Xp22.33. The P2RY8 Break Apart FISH probe is designed to label both ends of the P2RY8 gene, respectively. A 1270 Kb orange probe is designed at the 3′ end of the P2RY8 gene, and a 1000 Kb green probe is designed at the 5′ end of the P2RY8 gene.

Detects P2RY8 rearrangements in BCR-ABL1-like ALL (Philadelphia chromosome-like acute lymphoblastic leukemia), particularly P2RY8-CRLF2 fusion-positive childhood acute lymphoblastic leukemia cases associated with increased relapse risk. Used to identify P2RY8/IGH translocations in B-ALL patients with aggressive clinical course and poor prognosis. Essential for targeted therapy selection in treatment-resistant ALL cases.