OliGlow™ oligonucleotide probe is developed based on the bioinformatics analysis of targeted genomic DNA fragment by removing repeats and non-specific sequences to design and synthesize uniformly sized oligo pools and is not restricted by the availability of BAC clones. The number of oligos for a particular set of probes varies from several hundred to thousands. A library of oligo probes for each gene target is established and fluorescently labeled.

Probes generated by this technique avoid the interference from highly repetitive sequences in the human genome, and have the characteristics of shorter length, excellent tissue penetration, high specificity, clear signal, and low background.

Particularly, these probes possess the advantages of detecting genetic variations of adjacent genes that are not possible when probes were developed from BAC clones.