The VHL/CEN3 probe is located on chromosome 3. A 460 kb probe covering the VHL gene region at 3p25.3 is labeled with an orange dye, and part of chromosome 3 (CEN3) is labeled with a green dye.

Detects VHL gene deletions in renal cell carcinomas, particularly clear cell type where 3p25 deletion is found in 57.2% of clear cell carcinomas. Used for diagnosing von Hippel-Lindau disease-associated tumors and distinguishing RCC subtypes. Loss of heterozygosity at chromosome 3p and VHL inactivation is the most frequent genetic change in conventional renal cell carcinomas.