The HER2/CEN17 probe is located on chromosome 17. A 400 Kb probe covering the HER2 gene region is labeled with an orange dye, and part of chromosome 17 (CEN17) is labeled with a green dye.

HER2 gene amplification and overexpression are detected in roughly 15-30% of breast carcinomas, correlating strongly with elevated recurrence rates and unfavorable clinical outcomes. Similar overexpression patterns are documented in ovarian malignancies, gastric tumors, and high-grade endometrial neoplasms, including uterine serous carcinoma.

The human epidermal growth factor receptor 2 (HER2) gene is located on chromosome 17q12 and is a member of the epidermal growth factor receptors (EGFR).

Accurate detection of HER2 protein expression and gene amplification is a prerequisite for the screening and effective prediction of patients receiving molecular targeted therapy with anti-HER2 monoclonal antibody, and it is very important for the clinical treatment and/or prognosis evaluation of breast cancer